wnt pathway inhibitor iwp-2  (STEMCELL Technologies Inc)

Journal: PLOS ONE

Article Title: In vitro fish mucosal surfaces producing mucin as a model for studying host-pathogen interactions

doi: 10.1371/journal.pone.0308609

Figure Lengend Snippet: Representative images of PAS/Alcian blue stained RTgill-W1 (A-E) and CHSE-214 (F-J) cells grown on polycarbonate membranes (M) at two (A and F) and four (B-E, G-J) weeks post-confluence (PC). (A and F) Non-treated cells harvested at 2 weeks post-confluence. (B and G) Non-treated cells harvested at four weeks post-confluence. (C and H) DAPT treated cells harvested at four weeks post-confluence. (D and I) IWP-2 treated cells harvested at four weeks post-confluence. (E and J) Cells treated with a combination of DAPT and IWP-2 harvested at four weeks post-confluence. Images were captured with an Eclipse 90i microscope (Nikon) and are representative of six replicates per treatment. Scale bar = 30 μm.

Article Snippet: Together with the basic cell culture medium control, we thus had four different set ups: 1) basic cell culture medium control, 2) addition of 10 μM of the γ-secretase inhibitor DAPT (Sigma-Aldrich, Darmstadt, Germany) to the basic cell culture medium, 3) addition of 2 μM of the Wnt pathway inhibitor IWP-2 (Stemcell Technologies, Cambridge, UK) to basic cell culture medium and 4) addition of 10 μM DAPT and 2 μM of IWP-2 to the basic cell culture medium.

Techniques: Staining, Microscopy